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Work on a flat surface. Have a watch or other timer ready before starting the test.
1. Let the semen stand for twenty (20) minutes.
Semen is too thick to be tested immediately after ejaculation, so you must wait at least 20 minutes for semen to become thin (liquefy). Samples may be tested up to 3 hours after collection. Discard sample if not tested within 3 hours and wait at least 48 hours to obtain a fresh sample to test.
2. Place all of the kit components on a flat surface within easy reach.
3. Place the Solution Bottle into the Solution Bottle Stand on the box.
Push your finger through the roundperforation located in the top left corner on
the front of the box. This will create a stand to hold the Solution Bottle. Unscrew the cap on the Solution Bottle to remove it, and place the bottle into the stand so it won't tip over.
4. Fill the Semen Transfer Device to the line with semen.
Open the foil pouch labeled 'Semen Transfer Device' and take it out to use. Remove the cap from the Semen Collection Cup and use the Semen Transfer Device to gently stir and mix the semen sample in the Semen Collection Cup. Next, insert the Semen Transfer Device into the semen sample avoiding any solid or sticky material.
Slowly pull the plunger to draw semen into the Semen Transfer Device until it reaches the black line on the stem.
Avoid getting air bubbles in the Semen Transfer Device. If this occurs, push the semen back out of the Semen Transfer Device and then draw semen into the Semen Transfer Device again. Make sure the semen fills the Semen Transfer Device just to the line on the stem. Add or remove semen until it matches the line on the Semen Transfer Device exactly.
5. Add semen from the Semen Transfer Device to the Solution Bottle.
Insert the Semen Transfer Device into the Solution Bottle and press the plunger of the Semen Transfer Device gently to add the semen to the Solution Bottle.
6. Replace the cap on the Solution Bottle and mix the semen with the solution.
Screw the cap back onto the Solution Bottle and mix the contents by gently turning the Solution Bottle upside down several times. Do not shake vigorously as this will cause bubbles.
7. Wait for 2 minutes.
Place the Solution Bottle back in the Solution Bottle Stand and leave it there for two minutes before proceeding to the next step.
8. Unscrew the tip from the Solution Bottle cap.
Remove the Solution Bottle from the Solution Bottle Stand. Unscrew the tip from the Solution Bottle cap.
9. Add five (5) drops from the Solution Bottle to the Sample Well of the SpermCheck® Vasectomy Device.
Lay the SpermCheck® Vasectomy Device face up on a flat surface. Hold the Solution Bottle straight up and down over the sample well of the device and squeeze gently to add exactly five (5) drops of Solution. The sample well is marked with an “S” on the SpermCheck® Vasectomy Device.
Do not add more or less than 5 drops to the SpermCheck® Vasectomy Device sample well.
10. Begin timing and wait seven (7) minutes.
Begin timing after adding the Solution to the sample well.
11. Read the Result at 7 Minutes. See Page 3 for the details of How to Read the Results. This will explain the meaning of your test result.
Do not read the test result earlier or wait longer than 7 minutes as this may produce an incorrect result.
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